Deep Eutectic Solvent-Based Liquid-Liquid Microextraction Method for Separation and Determination of Tartrazine in Food Samples

Abstract

Tartrazine is an azo central composite design (CCD) [3]. In LLME-DES method, a certain amount of tartrazine was transferred to a 10 mL test tube. The pH of the analyte solution was adjusted to 4.5 by HCl and NaOH. Then, 450 μL of DES andye used in soft drinks, jellies, juices and other food products. It can cause many health problems including hyperactivity in children, allergies, migraine, asthma and eczema. Therefore, the determination of tartrazine in food samples in order to control and prevent the mentioned complications is very important. This study reports the application of deep eutectic solvents (DES), as a new generation of environmentally friendly solvents for liquid-liquid microextraction (LLME) of tartrazine in food samples [1,2]. DES was formed by mixing of choline chloride and phenol with 1:4 molar ratio. Four parameters of pH, DES volume, ultrasonic time and amount of salt were selected as effective factors on the process and optimized by d 1.2 g salt (NaCl) was added to the test tube to perform the extraction process. The solution was placed in an ultrasonic bath at 45°C for 16 min. After centrifugation for 15 minutes, two aqueous and DES-rich phases were formed. The aqueous phase was removed using a syringe and the DES-rich phase remaining at the end of the test tube was diluted with 0.5 mL of ethanol to be measured by UV-Vis spectrophotometry at 423 nm. The analytical curve obtained for tartrazine was in the linear concentration range of 10 to 600 ng mL-1, with limit of detection 2.89 ng mL-1 and the relative standard deviation (RSD) 4%. The proposed method was successfully applied for preconcentration and determination of tartrazine in food samples.