Bottom-up Proteomics: Identification of Salivary Gland Proteins in the Bishop's Mitre Shieldbug, Aelia Acuminat

Abstract

Proteomics is a powerful technique to identify proteins as important biomacromolecules. The determination of protein maps in the different organs using proteomics is the first step of molecular studies in the nutrition process. The salivary glands of Hemiptera are the main resource of secreting various proteins in the extra-oral digestion as a preliminary digestive process that occurs in the Hemiptera as specialty. The importance of salivary proteins is not only in the nutrition process but also they are key agents to elicit/suppress plant defense pathways in the insect-plant interaction. In this study, proteome map of salivary glands in the Aelia acuminata was visualized and some of the up-regulated proteins were identified using bottom-up proteomics. Final results lead to the identification of twenty-three proteins in the salivary gland tissues. Identified proteins belong to different categories contain digestive enzymes and their inhibitors, cell metabolism proteins, and cell defense proteins. Results indicate that the many known proteins in this research can be conside red appropriate candidates for use in enzyme-engineering programs to produce and develop new protein inhibitors in the wheat structure that leads to disruption of the feeding process in the bishop's mitre shieldbug.