Authors | Rouhollah Khani |
---|---|
Journal | Food Chemistry |
Page number | 220-225 |
Serial number | 293 |
IF | 4.529 |
Paper Type | Full Paper |
Published At | 2019 |
Journal Grade | ISI |
Journal Type | Typographic |
Journal Country | Iran, Islamic Republic Of |
Journal Index | JCR،Scopus |
Abstract
This study was conducted for quantitative determination of quercetin in food and fruit juice samples based on a green, fast, and accurate method namely, micro-cloud point extraction (MCPE). The proposed MCPE is essentially a miniaturized form of traditional cloud point extraction (CPE) in which only a few microliters of micellar extracting phase is suffient for determination. Factors inflencing the extraction effiency, such as amount of Triton X-114 (5%, v/v), effct of pH, amount of Na2SO4 salt (5%, w/v) and time of centrifugation were investigated and optimized by experimental design. Under the optimized condition, the calibration range was found to be linear over 10–100 ng mL−1 with the limit of detection of 2.2 ng mL−1. The relative standard deviation of 2.8%, was obtained on a 30 ng mL−1quercetin standard solution (n = 3). Finally, the proposed method was successfully applied for the determination of quercetin in onion, tomato, apple and orange juice samples.
tags: Quercetin Flavonoid Micro-cloud point extraction Spectrophotometry Experimental design