The effect of tannase-producing supplementation with probiotic potential on ruminal fermentation of diets containing pomegranate peel in vitro

Abstract

Due to the harmful effects of tannin on diet digestibility, the present study investigated the effect of tannase-producing supplementations (TPS) with probiotic potential on ruminal fermentation of diets containing pomegranate peel (PP) in vitro. The treatments consisted of a) basal diet (BD), b) BD+ 1 ml of TPS, c) BD+ 2 ml of TPS, and d) BD + 16% of PP, e) BD+ 1 ml of TPS+16% of PP and f) BD+ 2 ml of TPS+16% of PP. Gas production (GP) technique was used to study the effects of TPS on diets containing PP. TPS was freshly prepared and injected into the vials. According to the findings, TPS significantly increased the gas production potential of diets containing PP and simultaneously reduced the lag phase (P<0.05), confirming the effectiveness of the tannase feed additive.