Effects of wheat bran replacement with pomegranate seed pulp on rumen fermentation, gas production, methanogen and protozoa populations of camel and goat rumen using competitive PCR technique: An in vitro study

Abstract

Background: Microbial populations in the rumen play an essential role in the degradation of Cellulosic dietary components and in providing nutrients to the host animal. Objective: This study aims to detect the effect of PSP on rumen fermentation, digestibility, and methanogens and the protozoa population (by competitive PCR) of the camel and goat rumen fluid. Materials and Methods: PSP was added to the experimental treatments and replaced by wheat bran (0, 5, and 10%). Rumen fluid was collected from three goats and two camels according to the similarity of sex, breed, origin, and time and used for three gas production studies. DNA extraction was performed by the RBB+c method, the ImageJ program calculated band intensities (target and competing DNA), and line gradients were plotted based on the number of copies and intensity. Results: Our result showed that Diets did not significantly affect the methanogen and protozoa population. Animal species affected microbial populations so that both populations in camels were less than goats. The production of gas and volatile fatty acids was not affected by diets. These two parameters and NH3 concentration and methane production in goats was higher than in camel. The pH digested dry matter, and microbial protein in camels was higher than in goats. Conclusions: Therefore, the competitive PCR technique is an effective method for enumerating Rumen microbiota. This supplementation can be considered a strategy to achieve performance and environmental benefits.