Geographical variation in seed germination and biochemical response of milk thistle (Silybum marianum) ecotypes exposed to osmotic and salinity stresses

نویسندگانHossein Hammami-Bijan Saadatian-Seied Amir Hossein Hosseini
نشریهIndustrial Crops and Products
شماره صفحات1-12
شماره سریال9266690
شماره مجلد152
ضریب تاثیر (IF)5.645
نوع مقالهFull Paper
تاریخ انتشار25/4/2020
رتبه نشریهISI
نوع نشریهالکترونیکی
کشور محل چاپاسترالیا
نمایه نشریهISI

چکیده مقاله

Milk thistle (Silybum marianum) is a valuable medicinal plant and is spread in different habitats over several
areas in Iran. However, no information exists about seed germination and its tolerance to osmotic and salinity
stresses of Iranian populations. Therefore, seed germination and seedling responses to osmotic and salinity
stresses were determined in six Milk thistle populations originated from different native habitats. The populations
were collected from six protected areas in Iran. Hence, two laboratory experiments were arranged separately
as a completely randomized factorial design to investigate the effect of osmotic and salinity stresses. In the
1 st and 2nd experiment, seeds of six Milk thistle populations were subjected to five osmotic stress levels (0, -0.3,
-0.6, -0.9, and -1.2 MPa) and five salinity stress levels (0, 3, 6, 9, and 12 dSm−1), respectively. Ghamsar-Barzak
ecotype which, has been harvested from a dry climate with the lowest mean annual precipitation and Golestan
National Park ecotype which has been harvested from a Mediterranean climate with the highest mean annual
precipitation among the protected areas had the highest and lowest tolerance to osmotic, respectively. Generally,
biochemical traits including MDA, Proline, DPPH-Scavenging (%), Soluble carbohydrate, and Total phenol were
improved by increasing in osmotic severity in all ecotypes. The response to salinity was higher than osmotic in
all ecotypes. However, Ghamsar-Barzak ecotype showed more salinity tolerance compared to other ecotypes.
Under salinity conditions, MDA, proline, DPPH-Scavenging (%), soluble carbohydrate, and total phenol increased
more compared to the control treatment. The highest tolerance to salinity and osmotic stresses was
observed in Ghamsar-Barzak ecotype that obtained its seed was from a dry climate with the lowest mean annual
precipitation and soil pH and EC are high. Hence, the results of this study demonstrated that habitat-correlated
variation in Milk thistle seed response to osmotic and salinity stresses. It seems that Ghamsar-Barzak ecotype
could be a proper genetic source for gene transfer aimed at breeding for higher tolerance cultivars against
osmotic and salinity stresses.

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