Genetic variation and cluster analysis of Narcissus tazetta L. genotypes using RAPD and ISSR markers

Abstract

To evaluate the genetic diversity of Iranian Narcissus genotypes (e.g., Shomal, Shastpar, Shahla, Yasuj, Shiraz-1, Shiraz-2, Kuchak-e-Atri, Dutch, Khosf-1, Khosf-2, Birjand, and Tabas), different RAPD and ISSR primers were examined at the Plant Breeding Laboratory of the Faculty of Agriculture, University of Birjand, Iran. In sum, RAPD and ISSR primers produced 189 and 80 high-resolution bands. The average values of PIC, Ht, Hs, Nm, DST, FDT, NA, Ne, H, and I indices were 0.287, 0.369, 0.089, 0.486, 0.279, 0.760, 1.952, 1.459, 0.282, 0.437 for RAPD markers and equal to 0.297, 0.380, 0.099, 0.524, 0.278, 0.732, 1.978, 1.495, 0.303, 0.467 for ISSR markers, respectively. The analysis of molecular variance based on the RAPD and ISSR markers showed 23 and 13 % variations for intra-populations and 77 % and 87 % changes for interpopulation variabilities, respectively. Cluster analysis based on the RAPD and ISSR markers grouped genotypes into four clusters. Based on RAPD and ISSR markers, the PCoA analysis also showed that the first three components justified equal to 96.9 % and 97.9 % of the total variance, respectively, indicating the dispersion of the primers used. In general, it was concluded that the genetic diversity of narcissus species could be employed for breeding programs.