Atorvastatindetermination,using a novel electrochemical sensor

چکیده مقاله

Atorvastatin is a lipid-lowering agent and a member of a family of compounds referred to as statins [1] and is used to treat conditions such as pulmonary sarcoidosis. Voltammetric techniques are desirable techniques for the analysis of drugs because of their low cost, high sensitivity, ease of operation and portability [2]. As the most attractive derivative of voltametric techniques, deferential pulse voltammetry (DPV) has been applied to the determination of trace amounts of heavy metals and drugs [3]. On the other hand, carbon paste electrodes (CPEs) offer advantages of easy construction and surfacerenewability, low costs, inertness, robustness, response stability, low Ohmic resistance, lack of an internal solution, and compatibility with various modifiers. Modification of CPEs can further enhance their surface properties and significantly enhance the target signals. The electrochemical experiments were performed using a Palm-Sens (EN 50,081–2) potentiostat, a PC for data acquisition and processing, and a threeelectrode cell composed of the NF@Ch-modified carbon paste electrode (mCPE) as the working electrode, a saturated calomel reference electrode and a platinum auxiliary electrode (Azar Electrode Co, Iran). The potential readings were made against the reference electrode. After synthetized, the modifier was characterized, using FT-IR, TEM, VSM, XRD etc. To investigate the applicability of the mCPEs to the determination of ATV in real samples, human urine and plasma samples were used. Given that the samples were free from ATV, various amounts of the analyte were spiked to the samples prior to analyses. The effects of different organic/inorganic ions and compounds usually co-existing with ATV on the DPV analysis of this compound were studied investigated. Reproducibility and stability of NF@Ch-mCPEs were investigated through 9 successive analyses of a 50.0 nM ATV solution using one mCPE and the relative standard deviation (R.S.D.) was determined as 4.3%. The limit of detection of the method and the linear range were 35 pM and 0.1-100 nM respectively.