Liquid-phase microextraction of thiamine using supramolecular solvent as a carrier for ferrofluid

چکیده مقاله

Background:Thiamine is very important to the brain, particularly in terms of emotional health and well being, and also is useful for focus and concentration. Its absence is associated with serious diseases like Beriberi and Wernicke–Korsakoff syndrome. For all these reasons, the development of a sensitive method for the determination of thiamine is of much interest. This work reports, application a liquid-liquid microextraction based on supramolecular solvent (LLME-SUPSs) as a carrier for ferrofluid for the extraction and determination of thiamine [1]. Methods: A solution containing appropriate amount of thiamine, placed in 10 mL centrifuge tube. The pH of the sample solution was preserved at 11, then 1 mL of ferrofluid, 1.2 ml of potassium hexacyanoferrate (III) and 0.2 gr of salt were injected to the solution and the mixture was vigorously shaken using a shaker for 20 min. Subsequently, a strong magnet was placed next to the side of the centrifuge tube to collect the ferrofluid and the sample solution was discarded simply by decanting the glass tube. After that, appropriate amount of methanol was added to the vial in order to separate the extractant from magnetic particles (MPs). Results: The effect of various variables (pH, ultrasonic mixing time, volume of ferrofluid and potassium hexacyanoferrate (III) and amount of salt) on the LLME - SUPSs was investigated and optimized by (RSM) and (CCD) [2-3]. Under the optimum experimental conditions, the calibration curves found to be linear in the range of 4–800 ng m with correlation coefficients 0.9921, the (LOD) and (RSD) were 0.95 ng m and %2, respectively (n=5). The mean extraction recovery was 96%. Conclusion:In this study, a liquid–liquid microextraction technique based on the application of a nanostructured ferrofluid was developed. The proposed extraction technique lacks tedious steps of conventional microextraction methods. Moreover, this method offers significant advantages such as simplicity of the extraction, short extraction time and good precision and high accuracy.