Introducing of ultrasonic assisted dispersive liquid phase micro-extraction with magnetic dispersive solid phase extraction as a new simple sample preparation method

Abstract

Background: Rapid population growth of the world has led to an increase in demand for fuel and the advancement of chemical industry, fertilizers, pesticides, and medicine to maintain and improve living standards. Agriculture industry has become one of the most advanced industries of the world. The advancement in this industry has led to creation of new pesticides [1-4]. Methods: A tandem extraction method based on ultrasonic assisted liquid phase micro-extraction coupled to dispersive μ-solid phase extraction was utilized for determination of atrazine at μg L-1 concentration levels. In the first step a liquid phase micro-extraction based on dispersion of organic solvent was used for the fast extraction of atrazine from aqueous solution and in the next step the extraction organic solvent was effectively collected from the solution without any requirement to centrifuge step by using C-18-magnetic nanoparticles. After elution of the extracted targets from the magnetic nanoparticles, atrazine concentration was monitored by HPLC-UV at μgL-1 level. Results: The influencing parameters in the pre-concentration step are: pH of solution, magnetic based sorbent amount (mg) and 1-octanol volume (μL). Selected data for factors that resulted by Box-Behnken design were: pH of sample: 9.0, solid phase amount: 22 mg and extraction solvent volume: 36 microliter. Relative standard deviation under optimized conditions by the hybrid extraction method was observed to be< 7 % and method detection limit for atrazine was 0.3μgL-1. Conclusion: In the current work an efficient hybrid extraction method based on ultrasonic assisted dispersive liquid phase micro-extraction tandem with dispersive μ-solid phase extraction assisted was prosperously used for trace monitoring of atrazine in environmental water samples.