CdSe quantum dots capped with a deep eutectic solvent as a fluorescent probe for copper(II) determination in various drinks

Abstract

The present study shows that copper(II) ions can be determined with a new fluorescent probe that is based on the use of CdSe quantum dots capped with deep eutectic solvent (DES-CdSe QDs). The capped QDs were prepared in aqueous phase by a onestep procedure under ambient atmosphere using selenium dioxide as a stable precursor for selenium, and ascorbic acid as nontoxic reducing agent. The deep eutectic solvent is composed of choline chloride and thioglycolic acid and acts as stabilizing and functionalizing agent. The fluorescent probe undergoes an increase in the fluorescence intensity (with excitation/emission wavelengths at 380/560 nm) in the presence of Cu(II). Other ions display no significant effect on fluorescence. The effects of sample pH value, concentration of buffer, and volume of QDs solution were optimized by response surface methodology using a Box-Behnken statistical design. Under the optimal conditions, the response of the probe is linear in the 10–600 nM Cu(II) concentration range, with a 5.3 nM limit of detection. This is lower than the allowable maximum Cu(II) concentration in drinking water. The relative standard deviation of the method for five replicate measurements of Cu(II) at a 100 nM concentration level is 2.0%. The probe was successfully applied to the determination of Cu(II) in various drinks.