Authors | Mahsa Najafzadeh,, |
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Journal | Iranian Journal of Pharmaceutical Research |
Page number | 73-80 |
Serial number | 16 |
Volume number | 3 |
IF | 0.51 |
Paper Type | Full Paper |
Published At | 2020 |
Journal Grade | ISI |
Journal Type | Typographic |
Journal Country | Iran, Islamic Republic Of |
Journal Index | JCR،isc،Scopus |
Abstract
Silymarin is a safe herbal medicine; however, it has some undesirable properties such as short half-life and poor aqueous solubility. To the best of our knowledge, this study is the first to report utilizing a dual-drug delivery system (DDDS) to enhance the release profile of silymarin from both micelles and hydrogels. In this experimental study, the release profile of micellar silymarin and micelle-hydrogel bounded silymarin during 21 days was examined using Knauer K2600A liquid chromatography. The calibration curve was plotted using the peak-areas of the silymarin at different concentrations. The RP-C18 column allowed a good separation of the components of standard silymarin. LOD and LOQ were 16.5 and 55.02 μg/ml, respectively. The in vitro release profiles of the two compounds showed a rapid release of silymarin, especially in the absence of hydrogel. The cumulative release graph revealed that the hydrogel-bound form has more constant release kinetics than the free micelle form; this means that the hydrogel-bound form may sustain for longer durations. In this study, a dual-drug delivery system based on hydrogel/micelle composites was introduced. The results showed that Puramatrix hydrogel plays an important role in the constant release of silymarin. Furthermore, the RP-HPLC method presented in this study can be used by other researchers to overcome the difficulties associated with the in-vitro separation and quantification of silymarin.
tags: Micelles Puramatrix hydrogel slow-release RP-HPLC Silymarin